› 4bh4

X-ray diffraction
1.9Å resolution

Haemagglutinin from a Transmissible Mutant H5 Influenza Virus in Complex with Avian Receptor Analogue 3'-SLN

Released:
DOI: 10.2210/pdb4bh4/pdb
PDB entry 4bh4 coloured by chain, front view.
PDB entry 4bh4 coloured by chain, side view.
PDB entry 4bh4 coloured by chain, top view.
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Primary publication:
Receptor binding by a ferret-transmissible H5 avian influenza virus.
Xiong X, Coombs PJ, Martin SR, Liu J, Xiao H, McCauley JW, Locher K, Walker PA, Collins PJ, Kawaoka Y, Skehel JJ, Gamblin SJ
Nature 497 392-6 (2013)
PMID: 23615615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177029 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.2 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5EP31 (Residues: 17-342; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 167 amino acids
Theoretical weight: 19.26 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5EP31 (Residues: 347-513; Coverage: 29%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: R32
Unit cell:
a: 101.744Å b: 101.744Å c: 329.929Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.223
Expression system: Spodoptera frugiperda

Quick links

Citations

40 review citations

Bat-derived influenza-like viruses H17N10 and H18N11.
Wu et al. (2014)
39 more

2 mentions without citation

New insights into influenza A specificity: an evolution of paradigms.
Ji et al. (2017)
1 more