› 4bhh

X-ray diffraction
3.4Å resolution

Crystal structure of tetramer of La Crosse virus nucleoprotein in complex with ssRNA

Released:
DOI: 10.2210/pdb4bhh/pdb
PDB entry 4bhh coloured by chain, front view.
PDB entry 4bhh coloured by chain, side view.
PDB entry 4bhh coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for encapsidation of genomic RNA by La Crosse Orthobunyavirus nucleoprotein.
Reguera J, Malet H, Weber F, Cusack S
Proc Natl Acad Sci U S A 110 7246-51 (2013)
PMID: 23589854

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138222 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: B, D, F, Z
Molecule details ›
Chains: B, D, F, Z
Length: 236 amino acids
Theoretical weight: 26.62 KDa
Source organism: La Crosse virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04873 (Residues: 1-235; Coverage: 100%)
Gene name: N
Sequence domains: Bunyavirus nucleocapsid (N) protein
Structure domains:
POLY-URIDINE 45-MER Chain: R
Molecule details ›
Chain: R
Length: 45 nucleotides
Theoretical weight: 13.73 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 82.95Å b: 86.46Å c: 90.19Å
α: 90° β: 106.63° γ: 90°
R-values:
R R work R free
0.201 0.198 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

17 review citations

Orthobunyaviruses: recent genetic and structural insights.
Elliott RM. (2014)
16 more

7 mentions without citation

Nucleocapsid Structure of Negative Strand RNA Virus.
Luo et al. (2020)
6 more