› 4bhq

X-ray diffraction
2.05Å resolution

Structure of the periplasmic domain of the PilN type IV pilus biogenesis protein from Thermus thermophilus

Released:
DOI: 10.2210/pdb4bhq/pdb
PDB entry 4bhq coloured by chain, front view.
PDB entry 4bhq coloured by chain, side view.
PDB entry 4bhq coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis.
Karuppiah V, Collins RF, Thistlethwaite A, Gao Y, Derrick JP
Proc Natl Acad Sci U S A 110 E4638-47 (2013)
PMID: 24218553

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PilN biogenesis protein dimerization domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 17.93 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SII9 (Residues: 49-207; Coverage: 77%)
Gene name: TTHA1380
Sequence domains: PilN biogenesis protein dimerization domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: I41
Unit cell:
a: 67.83Å b: 67.83Å c: 147.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.226
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives.
Berry et al. (2015)
6 more

3 mentions without citation

Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis.
Karuppiah et al. (2013)
2 more