› 4bhr

X-ray diffraction
1.7Å resolution

Structure of the TTHA1221 type IV pilin protein from Thermus thermophilus

Released:
DOI: 10.2210/pdb4bhr/pdb
PDB entry 4bhr coloured by chain, front view.
PDB entry 4bhr coloured by chain, side view.
PDB entry 4bhr coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis.
Karuppiah V, Collins RF, Thistlethwaite A, Gao Y, Derrick JP
Proc Natl Acad Sci U S A 110 E4638-47 (2013)
PMID: 24218553

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pilin A4 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 10.13 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIZ3 (Residues: 37-122; Coverage: 71%)
Gene name: TTHA1221
Sequence domains: Pilin A4
Structure domains: Pantoate--beta-alanine Ligase; Chain: A,domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 36.79Å b: 36.79Å c: 207.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.216 0.241
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives.
Berry et al. (2015)
6 more

4 mentions without citation

Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis.
Karuppiah et al. (2013)
3 more