› 4bhu

X-ray diffraction
1.91Å resolution

Crystal structure of BslA - A bacterial hydrophobin

Released:
DOI: 10.2210/pdb4bhu/pdb
PDB entry 4bhu coloured by chain, front view.
PDB entry 4bhu coloured by chain, side view.
PDB entry 4bhu coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Primary publication:
BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm.
Hobley L, Ostrowski A, Rao FV, Bromley KM, Porter M, Prescott AR, MacPhee CE, van Aalten DM, Stanley-Wall NR
Proc Natl Acad Sci U S A 110 13600-5 (2013)
PMID: 23904481

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159766 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Biofilm-surface layer protein A Chains: A, B, C, D, E, F, G, H, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, J
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Escherichia coli B
UniProt:
  • Canonical: P71014 (Residues: 48-172; Coverage: 82%)
Gene names: BSU31080, bslA, yuaB
Sequence domains: Biofilm surface layer A
Structure domains: Immunoglobulin-like
Biofilm-surface layer protein A Chain: I
Molecule details ›
Chain: I
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10
Expression system: Escherichia coli B
UniProt:
  • Canonical: P71014 (Residues: 48-172; Coverage: 82%)
Gene names: BSU31080, bslA, yuaB
Sequence domains: Biofilm surface layer A
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand GOL 6 x GOL
Ligand CL 10 x CL
2 modified residues:
Ligand MLY 30 x MLY
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 69.7Å b: 95.98Å c: 259.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.199
Expression system: Escherichia coli B

Quick links

Citations

19 review citations

Biofilm formation by Bacillus subtilis: new insights into regulatory strategies and assembly mechanisms.
Cairns et al. (2014)
18 more

8 mentions without citation

Living together in biofilms: the microbial cell factory and its biotechnological implications.
Berlanga et al. (2016)
7 more