› 4biu

X-ray diffraction
3.65Å resolution

Crystal structure of CpxAHDC (orthorhombic form 1)

Released:
DOI: 10.2210/pdb4biu/pdb
PDB entry 4biu coloured by chain, front view.
PDB entry 4biu coloured by chain, side view.
PDB entry 4biu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation.
Mechaly AE, Sassoon N, Betton JM, Alzari PM
PLoS Biol 12 e1001776 (2014)
PMID: 24492262

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor histidine kinase CpxA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 298 amino acids
Theoretical weight: 33.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE82 (Residues: 188-457; Coverage: 59%)
Gene names: JW3882, b3911, cpxA, ecfB, eup, ssd
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 143.274Å b: 191.697Å c: 205.013Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.227
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
6 more

12 mentions without citation

The accommodation index measures the perturbation associated with insertions and deletions in coiled-coils: Application to understand signaling in histidine kinases.
Schmidt et al. (2017)
11 more