› 4bk0

X-ray diffraction
1.9Å resolution

Crystal structure of the KIX domain of human RECQL5 (domain-swapped dimer)

Released:
DOI: 10.2210/pdb4bk0/pdb
PDB entry 4bk0 coloured by chain, front view.
PDB entry 4bk0 coloured by chain, side view.
PDB entry 4bk0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5.
Kassube SA, Jinek M, Fang J, Tsutakawa S, Nogales E
Nat Struct Mol Biol 20 892-9 (2013)
PMID: 23748380

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase Q5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O94762 (Residues: 515-620; Coverage: 11%)
Gene names: RECQ5, RECQL5
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 50.67Å b: 61.21Å c: 81.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.234
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Transcription as a Threat to Genome Integrity.
Gaillard et al. (2016)
10 more

3 mentions without citation

Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5.
Kassube et al. (2013)
2 more