› 4bl7

X-ray diffraction
1.89Å resolution

Crystal structure of the AIMP3-MRS N-terminal domain complex in different space group

Released:
DOI: 10.2210/pdb4bl7/pdb
PDB entry 4bl7 coloured by chain, front view.
PDB entry 4bl7 coloured by chain, side view.
PDB entry 4bl7 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cho HY, Seo WW, Cho HJ, Kang BS

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128904 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methionine--tRNA ligase, cytoplasmic Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56192 (Residues: 1-224; Coverage: 25%)
Gene names: MARS, MARS1
Sequence domains:
Structure domains:
Eukaryotic translation elongation factor 1 epsilon-1 Chain: B
Molecule details ›
Chain: B
Length: 186 amino acids
Theoretical weight: 21.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43324 (Residues: 1-174; Coverage: 100%)
Gene names: AIMP3, EEF1E1, P18
Sequence domains: Glutathione S-transferase, C-terminal domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Unit cell:
a: 43.685Å b: 72.029Å c: 123.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.18 0.229
Expression system: Escherichia coli BL21(DE3)

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