› 4bme

X-ray diffraction
2Å resolution

Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant

Released:
DOI: 10.2210/pdb4bme/pdb
PDB entry 4bme coloured by chain, front view.
PDB entry 4bme coloured by chain, side view.
PDB entry 4bme coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Natural single amino acid polymorphism (F19Y) in human galectin-8: detection of structural alterations and increased growth-regulatory activity on tumor cells.
Ruiz FM, Scholz BA, Buzamet E, Kopitz J, André S, Menéndez M, Romero A, Solís D, Gabius HJ
FEBS J 281 1446-1464 (2014)
PMID: 24418318

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00214 (Residues: 4-155; Coverage: 48%)
Gene name: LGALS8
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 55.361Å b: 66.645Å c: 83.997Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.241
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Lectins: a primer for histochemists and cell biologists.
Manning et al. (2017)
7 more

2 mentions without citation

Natural single amino acid polymorphism (F19Y) in human galectin-8: detection of structural alterations and increased growth-regulatory activity on tumor cells.
Ruiz et al. (2014)
1 more