› 4bqf

X-ray diffraction
2.35Å resolution

Arabidopsis thaliana cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with acarbose

Released:
DOI: 10.2210/pdb4bqf/pdb
PDB entry 4bqf coloured by chain, front view.
PDB entry 4bqf coloured by chain, side view.
PDB entry 4bqf coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Sugar-Coated Sensor Chip and Nanoparticle Surfaces for the in Vitro Enzymatic Synthesis of Starch-Like Materials
O'Neill EC, Rashid AM, Stevenson CEM, Hetru AC, Gunning AP, Rejzek M, Nepogodiev SA, Bornemann S, Lawson DM, Field RA
Chem Sci 5 341- (2014)

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-glucan phosphorylase 2, cytosolic Chains: A, B
Molecule details ›
Chains: A, B
Length: 874 amino acids
Theoretical weight: 99.06 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SD76 (Residues: 1-841; Coverage: 100%)
Gene names: At3g46970, F13I12.20, PHS2
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
Carbohydrate polymer : NEW Components: GLC, BGC, AC1
Ligand GLC 2 x GLC
Ligand BGC 2 x BGC
Ligand AC1 2 x AC1
2 bound ligands:
Ligand GLC 3 x GLC
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 84.249Å b: 117.075Å c: 94.967Å
α: 90° β: 106.79° γ: 90°
R-values:
R R work R free
0.187 0.184 0.24
Expression system: Escherichia coli BL21(DE3)

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