› 4brz

X-ray diffraction
1.67Å resolution

Haloalkane dehalogenase

Released:
DOI: 10.2210/pdb4brz/pdb
PDB entry 4brz coloured by chain, front view.
PDB entry 4brz coloured by chain, side view.
PDB entry 4brz coloured by chain, top view.
Source organism: Rhodobacteraceae
Primary publication:
Biochemical and structural characterisation of a haloalkane dehalogenase from a marine Rhodobacteraceae.
Novak HR, Sayer C, Isupov MN, Gotz D, Spragg AM, Littlechild JA
FEBS Lett 588 1616-22 (2014)
PMID: 24613925

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 32.56 KDa
Source organism: Rhodobacteraceae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A067XG63 (Residues: 1-290; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 54.97Å b: 75.52Å c: 64.87Å
α: 90° β: 92.94° γ: 90°
R-values:
R R work R free
0.207 0.205 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
3 more