› 4bsa

X-ray diffraction
2.3Å resolution

Crystal Structure of the Haemagglutinin (with Asn-133 Glycosylation) from an H7N9 Influenza Virus Isolated from Humans

Released:
DOI: 10.2210/pdb4bsa/pdb
PDB entry 4bsa coloured by chain, front view.
PDB entry 4bsa coloured by chain, side view.
PDB entry 4bsa coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Receptor binding by an H7N9 influenza virus from humans.
Xiong X, Martin SR, Haire LF, Wharton SA, Daniels RS, Bennett MS, McCauley JW, Collins PJ, Walker PA, Skehel JJ, Gamblin SJ
Nature 499 496-9 (2013)
PMID: 23787694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126034 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.04 KDa
Source organism: Influenza A virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: M4YV75 (Residues: 19-339; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.44 KDa
Source organism: Influenza A virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: M4YV75 (Residues: 340-516; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 4 x NAG
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: R32
Unit cell:
a: 116.13Å b: 116.13Å c: 295.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.25 0.296
Expression system: Spodoptera frugiperda

Quick links

Citations

49 review citations

Role of receptor binding specificity in influenza A virus transmission and pathogenesis.
de Graaf et al. (2014)
48 more

2 mentions without citation

Analysis of recombinant H7N9 wild-type and mutant viruses in pigs shows that the Q226L mutation in HA is important for transmission.
Liu et al. (2014)
1 more