› 4buh

X-ray diffraction
1.3Å resolution

Human IgE against the major allergen Bet v 1 - Crystal structure of clone M0418 scFv

Released:
DOI: 10.2210/pdb4buh/pdb
Model geometry
Fit model/data
PDB entry 4buh coloured by chain, front view.
PDB entry 4buh coloured by chain, side view.
PDB entry 4buh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human IgE against the major allergen Bet v 1--defining an epitope with limited cross-reactivity between different PR-10 family proteins.
Levin M, Davies AM, Liljekvist M, Carlsson F, Gould HJ, Sutton BJ, Ohlin M
OpenAccess logo Clin Exp Allergy 44 288-99 (2014)
PMID: 24447087

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CLONE M0418 SCFV Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 28.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand PEG 1 x PEG
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 99.495Å b: 76.42Å c: 70.181Å
α: 90° β: 118.07° γ: 90°
R-values:
R R work R free
0.147 0.145 0.173
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The human IgE repertoire.
Gadermaier et al. (2014)
1 more

5 mentions without citation

Antibody Heavy Chain Variable Domains of Different Germline Gene Origins Diversify through Different Paths.
Kirik et al. (2017)
4 more