› 4bur

X-ray diffraction
2.88Å resolution

Crystal structure of the reduced human Apoptosis inducing factor complexed with NAD

Released:
DOI: 10.2210/pdb4bur/pdb
PDB entry 4bur coloured by chain, front view.
PDB entry 4bur coloured by chain, side view.
PDB entry 4bur coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the coenzyme mediated monomer-dimer transition of the pro-apoptotic apoptosis inducing factor.
Ferreira P, Villanueva R, Martínez-Júlvez M, Herguedas B, Marcuello C, Fernandez-Silva P, Cabon L, Hermoso JA, Lostao A, Susin SA, Medina M
Biochemistry 53 4204-15 (2014)
PMID: 24914854

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 511 amino acids
Theoretical weight: 55.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95831 (Residues: 103-613; Coverage: 83%)
Gene names: AIF, AIFM1, PDCD8
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAD 8 x NAD
Ligand FAD 4 x FAD
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 120.766Å b: 120.766Å c: 343.359Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.176 0.23
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Key Targets for Multi-Target Ligands Designed to Combat Neurodegeneration.
Ramsay et al. (2016)
4 more

8 mentions without citation

A novel AIFM1 mutation expands the phenotype to an infantile motor neuron disease.
Diodato et al. (2016)
7 more