› 4bvj

X-ray diffraction
1.6Å resolution

Function and Biology Details

Reaction catalysed: 
((R)-3-hydroxybutanoate)(n) + H(2)O = ((R)-3-hydroxybutanoate)(n-1 to 5) + ((R)-3-hydroxybutanoate)(1-5)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188027 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase (Class 2) Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 36.26 KDa
Source organism: Paucimonas lemoignei
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q939Q9 (Residues: 39-380; Coverage: 90%)
Gene names: EDC30_107150, phaZ7
Sequence domains: Lipase (class 2)
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 41.37Å b: 200.05Å c: 44.18Å
α: 90° β: 114.28° γ: 90°
R-values:
R R work R free
0.157 0.155 0.189
Expression system: Bacillus subtilis

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Citations

3 review citations

A holistic view of polyhydroxyalkanoate metabolism in Pseudomonas putida.
Prieto et al. (2016)
2 more