› 4bwz

X-ray diffraction
2.98Å resolution

Crystal structure of the sodium proton antiporter, NapA

Released:
DOI: 10.2210/pdb4bwz/pdb
PDB entry 4bwz coloured by chain, front view.
PDB entry 4bwz coloured by chain, side view.
PDB entry 4bwz coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
A two-domain elevator mechanism for sodium/proton antiport.
Lee C, Kang HJ, von Ballmoos C, Newstead S, Uzdavinys P, Dotson DL, Iwata S, Beckstein O, Cameron AD, Drew D
Nature 501 573-7 (2013)
PMID: 23995679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cation/H+ exchanger domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 41.23 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72IM4 (Residues: 1-386; Coverage: 100%)
Gene name: TT_C1108
Sequence domains: Sodium/hydrogen exchanger family
Structure domains: Na+/H+ antiporter like fold

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2221
Unit cell:
a: 73.75Å b: 82.15Å c: 191.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

31 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
30 more

18 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
17 more