› 4bx8

X-ray diffraction
2.4Å resolution

Human Vps33A

Released:
DOI: 10.2210/pdb4bx8/pdb
PDB entry 4bx8 coloured by chain, front view.
PDB entry 4bx8 coloured by chain, side view.
PDB entry 4bx8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.
Graham SC, Wartosch L, Gray SR, Scourfield EJ, Deane JE, Luzio JP, Owen DJ
Proc Natl Acad Sci U S A 110 13345-50 (2013)
PMID: 23901104

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188363 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 33A Chains: A, B
Molecule details ›
Chains: A, B
Length: 607 amino acids
Theoretical weight: 69.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96AX1 (Residues: 1-596; Coverage: 100%)
Gene name: VPS33A
Sequence domains: Sec1 family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 71.47Å b: 125.01Å c: 83.28Å
α: 90° β: 115.33° γ: 90°
R-values:
R R work R free
0.199 0.197 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Chaperoning SNARE assembly and disassembly.
Baker et al. (2016)
13 more

1 mention without citation

Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.
Graham et al. (2013)