› 4bxh

X-ray diffraction
2.24Å resolution

Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase

Released:
DOI: 10.2210/pdb4bxh/pdb
PDB entry 4bxh coloured by chain, front view.
PDB entry 4bxh coloured by chain, side view.
PDB entry 4bxh coloured by chain, top view.
Source organism: Flaveria trinervia
Primary publication:
Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase.
Schlieper D, Förster K, Paulus JK, Groth G
Mol Plant 7 437-40 (2014)
PMID: 24043710

Function and Biology Details

Reaction catalysed: 
Phosphate + oxaloacetate = H(2)O + phosphoenolpyruvate + HCO(3)(-)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151791 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C4 phosphoenolpyruvate carboxylase Chain: A
Molecule details ›
Chain: A
Length: 990 amino acids
Theoretical weight: 113.49 KDa
Source organism: Flaveria trinervia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30694 (Residues: 1-966; Coverage: 100%)
Gene name: PPCA
Sequence domains: Phosphoenolpyruvate carboxylase
C4 phosphoenolpyruvate carboxylase Chain: B
Molecule details ›
Chain: B
Length: 990 amino acids
Theoretical weight: 113.48 KDa
Source organism: Flaveria trinervia
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30694 (Residues: 1-966; Coverage: 100%)
Gene name: PPCA
Sequence domains: Phosphoenolpyruvate carboxylase

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 162.918Å b: 122.857Å c: 131.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism.
Yang et al. (2017)
4 more

2 mentions without citation

Identification of the allosteric site for neutral amino acids in the maize C4 isozyme of phosphoenolpyruvate carboxylase: The critical role of Ser-100.
González-Segura et al. (2018)
1 more