› 4bxl

Solution NMR

Structure of alpha-synuclein in complex with an engineered binding protein

Released:
DOI: 10.2210/pdb4bxl/pdb
PDB entry 4bxl coloured by chain, ensemble of 10 models, front view.
PDB entry 4bxl coloured by chain, ensemble of 10 models, side view.
PDB entry 4bxl coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
Sequestration of a ��-Hairpin for Control of ��-Synuclein Aggregation.
Mirecka EA, Shaykhalishahi H, Gauhar A, Akg��l S, Lecher J, Willbold D, Stoldt M, Hoyer W
Angew. Chem. Int. Ed. Engl. 53 4227-30 (2014)
PMID: 24623599

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153499 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AS69 Chains: A, B
Molecule details ›
Chains: A, B
Length: 46 amino acids
Theoretical weight: 5.09 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulin FC, subunit C
Alpha-synuclein Chain: C
Molecule details ›
Chain: C
Length: 22 amino acids
Theoretical weight: 2.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37840 (Residues: 35-56; Coverage: 16%)
Gene names: NACP, PARK1, SNCA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Affibody Molecules in Biotechnological and Medical Applications.
Ståhl et al. (2017)
9 more

8 mentions without citation

Modulating the Amyloidogenesis of α-Synuclein.
Sivanesam et al. (2016)
7 more