› 4bzk

Electron Microscopy
40Å resolution

The structure of the COPII coat assembled on membranes

Released:
DOI: 10.2210/pdb4bzk/pdb
PDB entry 4bzk coloured by chain, front view.
PDB entry 4bzk coloured by chain, side view.
PDB entry 4bzk coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structure of the COPII transport-vesicle coat assembled on membranes.
Zanetti G, Prinz S, Daum S, Meister A, Schekman R, Bacia K, Briggs JA
Elife 2 e00951 (2013)
PMID: 24062940
Related structures: EMD-2431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153784 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein transport protein SEC31 Chains: A, C
Molecule details ›
Chains: A, C
Length: 1273 amino acids
Theoretical weight: 138.83 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38968 (Residues: 1-1273; Coverage: 100%)
Gene names: D1229, SEC31, WEB1, YDL195W
Sequence domains:
Protein transport protein SEC13 Chain: B
Molecule details ›
Chain: B
Length: 297 amino acids
Theoretical weight: 33.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04491 (Residues: 1-297; Coverage: 100%)
Gene names: ANU3, L8167.4, SEC13, YLR208W
Sequence domains: WD domain, G-beta repeat
Protein transport protein SEC13 Chain: F
Molecule details ›
Chain: F
Length: 297 amino acids
Theoretical weight: 33.19 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04491 (Residues: 1-297; Coverage: 100%)
Gene names: ANU3, L8167.4, SEC13, YLR208W
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 40Å
Relevant EMDB volumes: EMD-2431
Expression system: Saccharomyces cerevisiae

Quick links

Citations

24 review citations

Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris.
Puxbaum et al. (2015)
23 more

5 mentions without citation

Vesicle trafficking and vesicle fusion: mechanisms, biological functions, and their implications for potential disease therapy.
Cui et al. (2022)
4 more