› 4bzw

X-ray diffraction
2.15Å resolution

Complete crystal structure of the carboxylesterase Cest-2923 (lp_2923) from Lactobacillus plantarum WCFS1

Released:
DOI: 10.2210/pdb4bzw/pdb
PDB entry 4bzw coloured by chain, front view.
PDB entry 4bzw coloured by chain, side view.
PDB entry 4bzw coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum WCFS1
Primary publication:
Structure, biochemical characterization and analysis of the pleomorphism of carboxylesterase Cest-2923 from Lactobacillus plantarum WCFS1.
Benavente R, Esteban-Torres M, Acebrón I, de Las Rivas B, Muñoz R, Alvarez Y, Mancheño JM
FEBS J 280 6658-71 (2013)
PMID: 24127688

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123792 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BD-FAE-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 31.28 KDa
Source organism: Lactiplantibacillus plantarum WCFS1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: F9US10 (Residues: 1-276; Coverage: 100%)
Gene name: lp_2923
Sequence domains: BD-FAE
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand SO4 9 x SO4
Ligand ACT 3 x ACT
Ligand 144 1 x 144
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6322
Unit cell:
a: 141.655Å b: 141.655Å c: 165.742Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.156 0.196
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation.
Khairul Anuar et al. (2022)
13 other articles

2 mentions without citation

Structural basis for dimerization and catalysis of a novel esterase from the GTSAG motif subfamily of the bacterial hormone-sensitive lipase family.
Li et al. (2014)
1 more