› 4c00

X-ray diffraction
2.25Å resolution

Crystal structure of TamA from E. coli

Released:
DOI: 10.2210/pdb4c00/pdb
PDB entry 4c00 coloured by chain, front view.
PDB entry 4c00 coloured by chain, side view.
PDB entry 4c00 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structural basis of autotransporter translocation by TamA.
Gruss F, Zähringer F, Jakob RP, Burmann BM, Hiller S, Maier T
Nat Struct Mol Biol 20 1318-20 (2013)
PMID: 24056943

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translocation and assembly module subunit TamA Chain: A
Molecule details ›
Chain: A
Length: 559 amino acids
Theoretical weight: 62.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADE4 (Residues: 22-577; Coverage: 100%)
Gene names: JW4179, b4220, tamA, yftM, ytfM
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MC3 16 x MC3
Ligand ACT 2 x ACT
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21212
Unit cell:
a: 77.47Å b: 261.06Å c: 57.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
26 more

17 mentions without citation

The β-barrel membrane protein insertase machinery from Gram-negative bacteria.
Noinaj et al. (2015)
16 more