› 4c0o

X-ray diffraction
2.56Å resolution

Transportin 3 in complex with phosphorylated ASF/SF2

Released:
DOI: 10.2210/pdb4c0o/pdb
PDB entry 4c0o coloured by chain, front view.
PDB entry 4c0o coloured by chain, side view.
PDB entry 4c0o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for nuclear import of splicing factors by human Transportin 3.
Maertens GN, Cook NJ, Wang W, Hare S, Gupta SS, Öztop I, Lee K, Pye VE, Cosnefroy O, Snijders AP, KewalRamani VN, Fassati A, Engelman A, Cherepanov P
Proc Natl Acad Sci U S A 111 2728-33 (2014)
PMID: 24449914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170574 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transportin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 923 amino acids
Theoretical weight: 104.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5L0 (Residues: 1-923; Coverage: 100%)
Gene names: IPO12, TNPO3
Sequence domains: Exportin 1-like protein
Serine/arginine-rich splicing factor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 125 amino acids
Theoretical weight: 14.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07955 (Residues: 106-230; Coverage: 50%)
Gene names: ASF, OK/SW-cl.3, SF2, SF2P33, SFRS1, SRSF1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
1 modified residue:
Ligand SEP 6 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P1
Unit cell:
a: 80.552Å b: 91.062Å c: 98.122Å
α: 106.98° β: 100.3° γ: 102.18°
R-values:
R R work R free
0.224 0.221 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
25 more

10 mentions without citation

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
9 more