› 4c1w

X-ray diffraction
1.84Å resolution

Carbohydrate binding domain from Streptococcus pneumoniae NanA sialidase complexed with 3'-sialyllactose

Released:
DOI: 10.2210/pdb4c1w/pdb
PDB entry 4c1w coloured by chain, front view.
PDB entry 4c1w coloured by chain, side view.
PDB entry 4c1w coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Prevention of influenza by targeting host receptors using engineered proteins.
Connaris H, Govorkova EA, Ligertwood Y, Dutia BM, Yang L, Tauber S, Taylor MA, Alias N, Hagan R, Nash AA, Webster RG, Taylor GL
Proc Natl Acad Sci U S A 111 6401-6 (2014)
PMID: 24733924

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
exo-alpha-sialidase Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.07 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4LB86 (Residues: 52-236; Coverage: 28%)
Gene name: nanA
Sequence domains: Sialidase, N-terminal domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 42.567Å b: 44.517Å c: 42.982Å
α: 90° β: 97° γ: 90°
R-values:
R R work R free
0.145 0.143 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Overview of the 3rd isirv-Antiviral Group Conference--advances in clinical management.
Hurt et al. (2015)
7 more

8 mentions without citation

Prevention of influenza by targeting host receptors using engineered proteins.
Connaris et al. (2014)
7 more