› 4c1y

X-ray diffraction
2.23Å resolution

Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with b-methylfucoside

Released:
DOI: 10.2210/pdb4c1y/pdb
PDB entry 4c1y coloured by chain, front view.
PDB entry 4c1y coloured by chain, side view.
PDB entry 4c1y coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Structural insights into Aspergillus fumigatus lectin specificity: AFL binding sites are functionally non-equivalent.
Houser J, Komarek J, Cioci G, Varrot A, Imberty A, Wimmerova M
Acta Crystallogr D Biol Crystallogr 71 442-53 (2015)
PMID: 25760594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-175846 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fucose-specific lectin Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 34.5 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4WW81 (Residues: 2-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin
Fucose-specific lectin Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 314 amino acids
Theoretical weight: 34.48 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4WW81 (Residues: 2-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin

Ligands and Environments

4 bound ligands:
Ligand MFB 12 x MFB
Ligand GOL 9 x GOL
Ligand PEG 1 x PEG
Ligand CA 1 x CA
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 71.211Å b: 90.335Å c: 189.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.243 0.313
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Histo-blood group antigens as mediators of infections.
Heggelund et al. (2017)
1 more

1 mention without citation

Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL.
Houser et al. (2017)