› 4c20

X-ray diffraction
2.41Å resolution

L-Fucose Isomerase

Released:
DOI: 10.2210/pdb4c20/pdb
PDB entry 4c20 coloured by chain, front view.
PDB entry 4c20 coloured by chain, side view.
PDB entry 4c20 coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Structural and functional analysis of fucose-processing enzymes from Streptococcus pneumoniae.
Higgins MA, Suits MD, Marsters C, Boraston AB
J Mol Biol 426 1469-82 (2014)
PMID: 24333485

Function and Biology Details

Reaction catalysed: 
L-fucopyranose = L-fuculose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188943 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-fucose isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 605 amino acids
Theoretical weight: 67.8 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97N97 (Residues: 2-588; Coverage: 100%)
Gene names: SP_2158, fucI
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand EDO 11 x EDO
Ligand SO4 4 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: R32
Unit cell:
a: 151.931Å b: 151.931Å c: 408.853Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.154 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
1 more

1 mention without citation

Enzymatic synthesis of l-fucose from l-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme.
Kim et al. (2019)