› 4c30

X-ray diffraction
3Å resolution

Crystal structure of Deinococcus radiodurans UvrD in complex with DNA, form 2

Released:
DOI: 10.2210/pdb4c30/pdb
PDB entry 4c30 coloured by chain, front view.
PDB entry 4c30 coloured by chain, side view.
PDB entry 4c30 coloured by chain, top view.
Source organisms:
Primary publication:
Structural and mechanistic insight into DNA unwinding by Deinococcus radiodurans UvrD.
Stelter M, Acajjaoui S, McSweeney S, Timmins J
PLoS One 8 e77364 (2013)
PMID: 24143224

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117275 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA 3'-5' helicase Chains: A, D, F, I
Molecule details ›
Chains: A, D, F, I
Length: 665 amino acids
Theoretical weight: 74.1 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RTI9 (Residues: 1-665; Coverage: 89%)
Gene name: DR_1775
Sequence domains:
Structure domains:
DNA STRAND FOR25 Chains: K, X
Molecule details ›
Chains: K, X
Length: 25 nucleotides
Theoretical weight: 7.68 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA STRAND REV25 Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 25 nucleotides
Theoretical weight: 7.62 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 68.489Å b: 89.785Å c: 293.798Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.231 0.228 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges.
Timmins et al. (2016)
2 more

5 mentions without citation

Grip it and rip it: structural mechanisms of DNA helicase substrate binding and unwinding.
Bhattacharyya et al. (2014)
4 more