› 4c4v

X-ray diffraction
3Å resolution

Structure of the outer membrane protein insertase BamA with one POTRA domain.

Released:
DOI: 10.2210/pdb4c4v/pdb
PDB entry 4c4v coloured by chain, front view.
PDB entry 4c4v coloured by chain, side view.
PDB entry 4c4v coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of BamA, an essential factor in outer membrane protein biogenesis.
Albrecht R, Schütz M, Oberhettinger P, Faulstich M, Bermejo I, Rudel T, Diederichs K, Zeth K
Acta Crystallogr D Biol Crystallogr 70 1779-89 (2014)
PMID: 24914988

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141798 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 52.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 347-810; Coverage: 59%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Structure domains:
Outer membrane protein assembly factor BamA Chain: B
Molecule details ›
Chain: B
Length: 467 amino acids
Theoretical weight: 52.47 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 344-810; Coverage: 59%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 79.583Å b: 67.347Å c: 109.795Å
α: 90° β: 93.46° γ: 90°
R-values:
R R work R free
0.236 0.234 0.292
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Outer Membrane Biogenesis.
Konovalova et al. (2017)
12 more

15 mentions without citation

The β-barrel assembly machinery in motion.
Noinaj et al. (2017)
14 more