4c5g

X-ray diffraction
2.1Å resolution

Crystal structure of the minimal Pho-Sfmbt complex (P6122 spacegroup)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-186100 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb protein Sfmbt Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 51.07 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VK33 (Residues: 531-980; Coverage: 37%)
Gene names: CG16975, Sfmbt
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.
Polycomb protein PHO Chain: B
Molecule details ›
Chain: B
Length: 32 amino acids
Theoretical weight: 3.63 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ST83 (Residues: 145-172; Coverage: 5%)
Gene names: CG17743, pho

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6122
Unit cell:
a: 155.1Å b: 155.1Å c: 100.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.237
Expression system: Escherichia coli BL21(DE3)