› 4c7g

X-ray diffraction
1.8Å resolution

Structure and activity of the GH20 beta-N-acetylhexosaminidase from Streptomyces coelicolor A3(2)

Released:
DOI: 10.2210/pdb4c7g/pdb
PDB entry 4c7g coloured by chain, front view.
PDB entry 4c7g coloured by chain, side view.
PDB entry 4c7g coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structure and activity of the Streptomyces coelicolor A3(2) β-N-acetylhexosaminidase provides further insight into GH20 family catalysis and inhibition.
Thi NN, Offen WA, Shareck F, Davies GJ, Doucet N
Biochemistry 53 1789-800 (2014)
PMID: 24559145

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-N-acetylhexosaminidase Chain: A
Molecule details ›
Chain: A
Length: 494 amino acids
Theoretical weight: 54.21 KDa
Source organism: Streptomyces coelicolor
Expression system: Streptomyces lividans
UniProt:
  • Canonical: Q9L068 (Residues: 42-535; Coverage: 99%)
Gene names: SCC105.17c, SCO2786, hexA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NGO 1 x NGO
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 58.17Å b: 64.45Å c: 143.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.21
Expression system: Streptomyces lividans

Quick links

Citations

1 review citation

α-Mannosidase and β-D-N-acetylhexosaminidase outside the wall: partner exoglycosidases involved in fruit ripening process.
Morales-Quintana et al. (2023)
7 other articles

1 mention without citation

Structural-Functional Analysis Reveals a Specific Domain Organization in Family GH20 Hexosaminidases.
Val-Cid et al. (2015)