› 4c97

X-ray diffraction
1.7Å resolution

Cas6 (TTHA0078) H37A mutant

Released:
DOI: 10.2210/pdb4c97/pdb
PDB entry 4c97 coloured by chain, front view.
PDB entry 4c97 coloured by chain, side view.
PDB entry 4c97 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.
Niewoehner O, Jinek M, Doudna JA
Nucleic Acids Res 42 1341-53 (2014)
PMID: 24150936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated protein Cas6 C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 26.51 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SM65 (Residues: 1-239; Coverage: 100%)
Gene name: TTHA0078
Sequence domains: CRISPR-associated endoribonuclease Cas6
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 73.93Å b: 77.45Å c: 88.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
van der Oost et al. (2014)
15 more

2 mentions without citation

Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.
Niewoehner et al. (2014)
1 more