› 4c98

X-ray diffraction
2Å resolution

Thermus thermophilus Cas6 (TTHB231)

Released:
DOI: 10.2210/pdb4c98/pdb
PDB entry 4c98 coloured by chain, front view.
PDB entry 4c98 coloured by chain, side view.
PDB entry 4c98 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.
Niewoehner O, Jinek M, Doudna JA
Nucleic Acids Res 42 1341-53 (2014)
PMID: 24150936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CAS6B Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 29.27 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53VU8 (Residues: 1-264; Coverage: 100%)
Gene name: TTHB231
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P41212
Unit cell:
a: 68.54Å b: 68.54Å c: 133.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
van der Oost et al. (2014)
15 more

2 mentions without citation

Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.
Niewoehner et al. (2014)
1 more