› 4c9d

X-ray diffraction
3Å resolution

Cas6 (TTHB231) product complex

Released:
DOI: 10.2210/pdb4c9d/pdb
PDB entry 4c9d coloured by chain, front view.
PDB entry 4c9d coloured by chain, side view.
PDB entry 4c9d coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Evolution of CRISPR RNA recognition and processing by Cas6 endonucleases.
Niewoehner O, Jinek M, Doudna JA
Nucleic Acids Res 42 1341-53 (2014)
PMID: 24150936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227917 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CAS6B Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 29.27 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53VU8 (Residues: 1-264; Coverage: 100%)
Gene name: TTHB231
Sequence domains:
Structure domains:
R3 REPEAT RNA CLEAVAGE PRODUCT Chains: C, D
Molecule details ›
Chains: C, D
Length: 29 nucleotides
Theoretical weight: 9.38 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 23G 2 x 23G

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P43212
Unit cell:
a: 75.22Å b: 75.22Å c: 308.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.291
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

15 review citations

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems.
Mohanraju et al. (2016)
14 more

4 mentions without citation

Unravelling the structural and mechanistic basis of CRISPR-Cas systems.
van der Oost et al. (2014)
3 more