› 4c9y

X-ray diffraction
2.01Å resolution

Structural Basis for the microtubule binding of the human kinetochore Ska complex

Released:
DOI: 10.2210/pdb4c9y/pdb
PDB entry 4c9y coloured by chain, front view.
PDB entry 4c9y coloured by chain, side view.
PDB entry 4c9y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for microtubule recognition by the human kinetochore Ska complex.
Abad MA, Medina B, Santamaria A, Zou J, Plasberg-Hill C, Madhumalar A, Jayachandran U, Redli PM, Rappsilber J, Nigg EA, Jeyaprakash AA
Nat Commun 5 2964 (2014)
PMID: 24413531

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spindle and kinetochore-associated protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 123 amino acids
Theoretical weight: 14.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96BD8 (Residues: 133-255; Coverage: 48%)
Gene names: C18orf24, SKA1
Sequence domains: Spindle and kinetochore-associated protein 1
Structure domains: Ska1 microtubule binding domain-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2221
Unit cell:
a: 39.006Å b: 161.578Å c: 104.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.194 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
10 more

2 mentions without citation

Structural basis for microtubule recognition by the human kinetochore Ska complex.
Abad et al. (2014)
1 more