› 4cad

X-ray diffraction
2.5Å resolution

Mechanism of farnesylated CAAX protein processing by the integral membrane protease Rce1

Released:
DOI: 10.2210/pdb4cad/pdb
PDB entry 4cad coloured by chain, front view.
PDB entry 4cad coloured by chain, side view.
PDB entry 4cad coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of farnesylated CAAX protein processing by the intramembrane protease Rce1.
Manolaridis I, Kulkarni K, Dodd RB, Ogasawara S, Zhang Z, Bineva G, Reilly NO, Hanrahan SJ, Thompson AJ, Cronin N, Iwata S, Barford D
Nature 504 301-5 (2013)
PMID: 24291792

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212383 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 214 amino acids
Theoretical weight: 23.66 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 227 amino acids
Theoretical weight: 24.37 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
CAAX prenyl protease 2 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 271 amino acids
Theoretical weight: 31.2 KDa
Source organism: Methanococcus maripaludis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6LZY8 (Residues: 1-271; Coverage: 100%)
Gene names: MMP0485, rce1
Sequence domains: Type II CAAX prenyl endopeptidase Rce1-like

Ligands and Environments

2 bound ligands:
Ligand BOG 2 x BOG
Ligand LMT 2 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 113.063Å b: 90.087Å c: 99.3Å
α: 89.06° β: 102.29° γ: 90°
R-values:
R R work R free
0.228 0.226 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Protein prenylation: unique fats make their mark on biology.
Wang et al. (2016)
24 more

10 mentions without citation

Rce1: mechanism and inhibition.
Hampton et al. (2018)
9 more