› 4cbu

X-ray diffraction
1.3Å resolution

Crystal structure of Plasmodium falciparum actin I

Released:
DOI: 10.2210/pdb4cbu/pdb
Model geometry
Fit model/data
PDB entry 4cbu coloured by chain, front view.
PDB entry 4cbu coloured by chain, side view.
PDB entry 4cbu coloured by chain, top view.
Source organisms:
Primary publication:
Structural differences explain diverse functions of Plasmodium actins.
Vahokoski J, Bhargav SP, Desfosses A, Andreadaki M, Kumpula EP, Martinez SM, Ignatev A, Lepper S, Frischknecht F, Sidén-Kiamos I, Sachse C, Kursula I
OpenAccess logo PLoS Pathog 10 e1004091 (2014)
PMID: 24743229

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146438 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-1 Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 42.05 KDa
Source organism: Plasmodium falciparum
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8I4X0 (Residues: 1-376; Coverage: 100%)
Gene names: ACT1, ACTI, PF3D7_1246200, PFL2215w
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 127 amino acids
Theoretical weight: 14.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13020 (Residues: 50-174; Coverage: 17%)
Gene names: Gsb, Gsn
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

2 bound ligands:
Ligand ATP 1 x ATP
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 68.98Å b: 110.3Å c: 71.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.12 0.153
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The Molecular Basis of Erythrocyte Invasion by Malaria Parasites.
Cowman et al. (2017)
7 more

11 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
10 more