› 4cdi

X-ray diffraction
3.7Å resolution

Crystal structure of AcrB-AcrZ complex

Released:
DOI: 10.2210/pdb4cdi/pdb
PDB entry 4cdi coloured by chain, front view.
PDB entry 4cdi coloured by chain, side view.
PDB entry 4cdi coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the AcrAB-TolC multidrug efflux pump.
Du D, Wang Z, James NR, Voss JE, Klimont E, Ohene-Agyei T, Venter H, Chiu W, Luisi BF
Nature 509 512-5 (2014)
PMID: 24747401

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151931 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:
PREDICTED PROTEIN Chain: C
Molecule details ›
Chain: C
Length: 49 amino acids
Theoretical weight: 5.3 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli BL21(DE3)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24, DIAMOND BEAMLINE I04-1
Spacegroup: R32
Unit cell:
a: 146.205Å b: 146.205Å c: 543.207Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.34 0.339 0.361
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

91 review citations

Molecular mechanisms of antibiotic resistance.
Blair et al. (2015)
90 more

5 mentions without citation

Structural Insights into Transporter-Mediated Drug Resistance in Infectious Diseases.
Kim et al. (2021)
4 more