› 4cek

X-ray diffraction
2.35Å resolution

Crystal structure of the second MIF4G domain of human nonsense mediated decay factor UPF2

Released:
DOI: 10.2210/pdb4cek/pdb
PDB entry 4cek coloured by chain, front view.
PDB entry 4cek coloured by chain, side view.
PDB entry 4cek coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.
Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S
Nucleic Acids Res 42 2673-86 (2014)
PMID: 24271394

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of nonsense transcripts 2 Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 35.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HAU5 (Residues: 455-757; Coverage: 24%)
Gene names: KIAA1408, RENT2, UPF2
Sequence domains: MIF4G domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 40.88Å b: 56.6Å c: 142.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story.
He et al. (2015)
12 more

3 mentions without citation

No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors.
Mailliot et al. (2022)
2 more