› 4cf4

X-ray diffraction
2.14Å resolution

Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase

Released:
DOI: 10.2210/pdb4cf4/pdb
Model geometry
Fit model/data
PDB entry 4cf4 coloured by chain, front view.
PDB entry 4cf4 coloured by chain, side view.
PDB entry 4cf4 coloured by chain, top view.
Source organism: Rhodobacteraceae
Entry authors: Novak HR, Sayer C, Isupov MN, Littlechild JA

Function and Biology Details

Reaction catalysed: 
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-haloacid dehalogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 25.76 KDa
Source organism: Rhodobacteraceae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: R4GRT2 (Residues: 1-236; Coverage: 100%)
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ASB 2 x ASB

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 46.09Å b: 86.64Å c: 105.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.202 0.28
Expression system: Escherichia coli BL21(DE3)

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