› 4cg3

X-ray diffraction
1.55Å resolution

Structural and functional studies on a thermostable polyethylene therephtalate degrading hydrolase from Thermobifida fusca

Released:
DOI: 10.2210/pdb4cg3/pdb
PDB entry 4cg3 coloured by chain, ensemble of 77 models, front view.
PDB entry 4cg3 coloured by chain, ensemble of 77 models, side view.
PDB entry 4cg3 coloured by chain, ensemble of 77 models, top view.
Source organism: Thermobifida fusca
Primary publication:
Structural and functional studies on a thermostable polyethylene terephthalate degrading hydrolase from Thermobifida fusca.
Roth C, Wei R, Oeser T, Then J, Föllner C, Zimmermann W, Sträter N
Appl Microbiol Biotechnol 98 7815-23 (2014)
PMID: 24728714

Function and Biology Details

Reactions catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase cut2 Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 33.79 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6A0I4 (Residues: 41-301; Coverage: 100%)
Gene names: TfH, bta1, cut1, cut2
Sequence domains: Chlorophyllase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: I41
Unit cell:
a: 117.9Å b: 117.9Å c: 36.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.117 0.149
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: how far are we?
Wei et al. (2017)
24 more

4 mentions without citation

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields.
Kawai et al. (2019)
3 more