› 4chc

X-ray diffraction
2.77Å resolution

Crystal structure of the N-terminal domain of the PA subunit of Thogoto virus polymerase (form 2)

Released:
DOI: 10.2210/pdb4chc/pdb
PDB entry 4chc coloured by chain, front view.
PDB entry 4chc coloured by chain, side view.
PDB entry 4chc coloured by chain, top view.
Source organism: Thogoto thogotovirus
Primary publication:
Comparative structural and functional analysis of orthomyxovirus polymerase cap-snatching domains.
Guilligay D, Kadlec J, Crépin T, Lunardi T, Bouvier D, Kochs G, Ruigrok RW, Cusack S
PLoS One 9 e84973 (2014)
PMID: 24454773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 177 amino acids
Theoretical weight: 20.5 KDa
Source organism: Thogoto thogotovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27194 (Residues: 1-170; Coverage: 27%)
Gene name: Segment 3
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 206Å b: 79.16Å c: 70.3Å
α: 90° β: 97.23° γ: 90°
R-values:
R R work R free
0.231 0.229 0.279
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Regulatory role of small nucleolar RNAs in human diseases.
Stepanov et al. (2015)
4 more