› 4chu

X-ray diffraction
2.49Å resolution

E. coli IscR-DNA complex

Released:
DOI: 10.2210/pdb4chu/pdb
PDB entry 4chu coloured by chain, front view.
PDB entry 4chu coloured by chain, side view.
PDB entry 4chu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium.
Santos JA, Alonso-García N, Macedo-Ribeiro S, Pereira PJ
Proc Natl Acad Sci U S A 111 E2251-60 (2014)
PMID: 24847070

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111208 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional regulator IscR Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 17.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3SZN7 (Residues: 2-162; Coverage: 99%)
Gene names: A5U30_000688, ACN81_26430, ACU57_06280, AW118_13225, AWP47_26715, B6R15_001681, BANRA_01076, BANRA_03397, BE932_10465, BER14_02660, BG944_001702, BGM66_000699, BGZ_03236, BGZ_03450, BJI68_01810, BK292_20655, BK383_22850, BKL28_000890, BTB68_002244, BXT93_17620, BvCmsKKP061_03887, C0P57_000057, C2121_002608, C2M16_05915, C2R31_001969, C3F40_24280, C9Z68_01855, CG704_04510, CG831_000287, CIG67_10155, CTR35_002286, D3C88_00800, D3G36_12880, D4M65_04155, D4N09_13930, D9D43_10020, D9E49_14250, D9J61_05885, DNQ45_01205, DNX30_07370, DS732_18780, DTL43_01805, DU321_15895, E4K51_06860, E6D34_12975, ECs3397, EIA08_10500, EIZ93_07240, EPS76_15870, EPS97_09460, EWK56_23230, ExPECSC038_01045, F7F11_13470, F7N46_01115, F9413_00835, F9461_12240, F9B07_15575, FGAF848_30010, FIJ20_06770, FJQ40_04385, FOI11_000295, FOI11_19755, FORC44_1305, FPI65_15805, FPS11_07495, FZU14_01845, G3V95_05475, G3W53_11630, G4A38_06605, G4A47_09555, G5603_17865, GAJ12_03350, GKF66_14440, GNW61_14595, GOP25_16490, GP711_05615, GP965_14665, GP979_16540, GQA06_18630, GQE86_05175, GQM04_00540, GQM21_00300, GQN34_09055, GQW07_09775, GRC73_00265, GRO95_08935, GRW05_03165, GRW24_30905, GRW56_08130, H0O72_14765, HEP30_006300, HEP34_002828, HI055_002134, HIE29_003801, HJQ60_001004, HLX92_08245, HLZ50_15730, HMV95_11165, HV109_06085, HV209_12295, HVV39_04680, HVW04_22385, HVW43_23685, I6H00_25350, I6H02_07960, IFB95_000455, J0541_000314, J5U05_001887, JNP96_20425, NCTC10082_03757, NCTC10089_01316, NCTC10429_02103, NCTC10764_00569, NCTC10767_02592, NCTC10974_01566, NCTC11112_07257, NCTC11126_06223, NCTC11181_03573, NCTC7922_00891, NCTC7927_01498, NCTC7928_01373, NCTC8009_03117, NCTC8179_04407, NCTC8333_01559, NCTC8500_01440, NCTC8621_01399, NCTC8959_02464, NCTC8985_06159, NCTC9001_01587, NCTC9044_00278, NCTC9045_01543, NCTC9073_04549, NCTC9075_01971, NCTC9077_01714, NCTC9081_05591, NCTC9117_01878, NCTC9702_01579, NCTC9706_04614, NCTC9962_00700, NEP60_15235, OGM49_18005, QDW62_06580, SAMEA3472112_00778, SAMEA3752557_00967, WR15_01185, iscR, iscR_1
Sequence domains: Iron-dependent Transcriptional regulator
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
HYA PROMOTER FRAGMENT Chain: C
Molecule details ›
Chain: C
Length: 27 nucleotides
Theoretical weight: 8.3 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
HYA PROMOTER FRAGMENT Chain: D
Molecule details ›
Chain: D
Length: 27 nucleotides
Theoretical weight: 8.29 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 49.015Å b: 75.835Å c: 173.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Fe-S proteins that regulate gene expression.
Mettert et al. (2015)
3 more

4 mentions without citation

Tangled web of interactions among proteins involved in iron-sulfur cluster assembly as unraveled by NMR, SAXS, chemical crosslinking, and functional studies.
Kim et al. (2015)
3 more