› 4cja

X-ray diffraction
2.65Å resolution

BurrH DNA-binding protein from Burkholderia rhizoxinica in complex with its target DNA

Released:
DOI: 10.2210/pdb4cja/pdb
PDB entry 4cja coloured by chain, front view.
PDB entry 4cja coloured by chain, side view.
PDB entry 4cja coloured by chain, top view.
Source organism: Mycetohabitans rhizoxinica
Primary publication:
BuD, a helix-loop-helix DNA-binding domain for genome modification.
Stella S, Molina R, López-Méndez B, Juillerat A, Bertonati C, Daboussi F, Campos-Olivas R, Duchateau P, Montoya G
Acta Crystallogr D Biol Crystallogr 70 2042-52 (2014)
PMID: 25004980

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117292 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
BURRH Chain: A
Molecule details ›
Chain: A
Length: 794 amino acids
Theoretical weight: 82.95 KDa
Source organism: Mycetohabitans rhizoxinica
Expression system: Escherichia coli BL21
5'-D(*DTP*TP*AP*AP*GP*AP*GP*AP*AP*GP*CP*AP*AP*DP *TP*AP*CP*GP*TP*TP*AP*TP*AP)-3' Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.12 KDa
Source organism: Mycetohabitans rhizoxinica
Expression system: Not provided
5'-D(*DTP*AP*TP*AP*AP*CP*GP*TP*AP*TP*TP*TP*GP*CP *TP*TP*CP*TP*CP*TP*TP*AP*AP)-3' Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7 KDa
Source organism: Mycetohabitans rhizoxinica
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 70.242Å b: 95.95Å c: 76.446Å
α: 90° β: 109.5° γ: 90°
R-values:
R R work R free
0.206 0.203 0.268
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

The genome editing revolution: A CRISPR-Cas TALE off-target story.
Stella et al. (2016)
3 more

5 mentions without citation

Leveraging self-assembled nanobiomaterials for improved cancer immunotherapy.
Vincent et al. (2022)
4 more