› 4clv

X-ray diffraction
3.12Å resolution

Crystal Structure of dodecylphosphocholine-solubilized NccX from Cupriavidus metallidurans 31A

Released:
DOI: 10.2210/pdb4clv/pdb
PDB entry 4clv coloured by chain, front view.
PDB entry 4clv coloured by chain, side view.
PDB entry 4clv coloured by chain, top view.
Source organism: Cupriavidus metallidurans
Primary publication:
The X-ray structure of NccX from Cupriavidus metallidurans 31A illustrates potential dangers of detergent solubilization when generating and interpreting crystal structures of membrane proteins.
Ziani W, Maillard AP, Petit-Härtlein I, Garnier N, Crouzy S, Girard E, Covès J
J Biol Chem 289 31160-72 (2014)
PMID: 25258316

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175031 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel-cobalt-cadmium resistance protein NccX Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 16.71 KDa
Source organism: Cupriavidus metallidurans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q44582 (Residues: 1-148; Coverage: 100%)
Gene name: nccX
Sequence domains: Heavy-metal resistance

Ligands and Environments

3 bound ligands:
Ligand ZN 10 x ZN
Ligand PO4 4 x PO4
Ligand PC 2 x PC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 53.8Å b: 53.8Å c: 300.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.251 0.264
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Perturbations of Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical Studies.
Chipot et al. (2018)
5 other articles

1 mention without citation

The X-ray structure of NccX from Cupriavidus metallidurans 31A illustrates potential dangers of detergent solubilization when generating and interpreting crystal structures of membrane proteins.
Ziani et al. (2014)