› 4cmr

X-ray diffraction
1.8Å resolution

The crystal structure of novel exo-type maltose-forming amylase(Py04_0872) from Pyrococcus sp. ST04

Released:
DOI: 10.2210/pdb4cmr/pdb
PDB entry 4cmr coloured by chain, front view.
PDB entry 4cmr coloured by chain, side view.
PDB entry 4cmr coloured by chain, top view.
Source organism: Pyrococcus sp. ST04
Primary publication:
Structural features underlying the selective cleavage of a novel exo-type maltose-forming amylase from Pyrococcus sp. ST04.
Park KH, Jung JH, Park SG, Lee ME, Holden JF, Park CS, Woo EJ
Acta Crystallogr D Biol Crystallogr 70 1659-68 (2014)
PMID: 24914977

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase/deacetylase family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 597 amino acids
Theoretical weight: 71.08 KDa
Source organism: Pyrococcus sp. ST04
Expression system: Escherichia coli
UniProt:
  • Canonical: I3RE04 (Residues: 1-597; Coverage: 100%)
Gene name: Py04_0872

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 34 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2221
Unit cell:
a: 117.639Å b: 370.794Å c: 82.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.225
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of α-glucan debranching enzymes.
Møller et al. (2016)
1 more