› 4cnm

X-ray diffraction
1.75Å resolution

Crystal structure of human 5T4 (Wnt-activated inhibitory factor 1, Trophoblast glycoprotein)

Released:
DOI: 10.2210/pdb4cnm/pdb
PDB entry 4cnm coloured by chain, front view.
PDB entry 4cnm coloured by chain, side view.
PDB entry 4cnm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the inhibition of Wnt signaling by cancer antigen 5T4/Wnt-activated inhibitory factor 1.
Zhao Y, Malinauskas T, Harlos K, Jones EY
Structure 22 612-20 (2014)
PMID: 24582434

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Trophoblast glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 32.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13641 (Residues: 60-345; Coverage: 74%)
Gene names: 5T4, TPBG
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 4 x NAG
Ligand PEG 5 x PEG
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 49.31Å b: 67.31Å c: 96.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Extracellular modulators of Wnt signalling.
Malinauskas et al. (2014)
6 more

2 mentions without citation

A highly stable human single-domain antibody-drug conjugate exhibits superior penetration and treatment of solid tumors.
Wu et al. (2022)
1 more