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› 4cqn

X-ray diffraction
2.5Å resolution

Crystal structure of the E.coli LeuRS-tRNA complex with the non- cognate isoleucyl adenylate analogue

Released:
DOI: 10.2210/pdb4cqn/pdb
Model geometry
Fit model/data
PDB entry 4cqn coloured by chain, front view.
PDB entry 4cqn coloured by chain, side view.
PDB entry 4cqn coloured by chain, top view.
Source organisms:
Primary publication:
The physiological target for LeuRS translational quality control is norvaline.
Cvetesic N, Palencia A, Halasz I, Cusack S, Gruic-Sovulj I
EMBO J 33 1639-53 (2014)
PMID: 24935946

Function and Biology Details

Reaction catalysed: 
tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-302112 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Leucine--tRNA ligase Chains: A, D
Molecule details ›
Chains: A, D
Length: 880 amino acids
Theoretical weight: 99.52 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07813 (Residues: 1-860; Coverage: 100%)
Gene names: JW0637, b0642, leuS
Sequence domains:
Structure domains:
ESCHERICHIA COLI TRNA-LEU UAA ISOACCEPTOR Chains: B, E
Molecule details ›
Chains: B, E
Length: 82 nucleotides
Theoretical weight: 26.48 KDa
Source organism: Escherichia coli
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ILA 4 x ILA
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 155.62Å b: 67.58Å c: 224.59Å
α: 90° β: 105° γ: 90°
R-values:
R R work R free
0.218 0.214 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Metabolite-Enzyme Coevolution: From Single Enzymes to Metabolic Pathways and Networks.
Noda-Garcia et al. (2018)
3 more

6 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
5 more