› 4cr0

X-ray diffraction
2.65Å resolution

Crystal Structure of H5 (VN1194) Asn186Lys/Gly143Arg Mutant Haemagglutinin

Released:
DOI: 10.2210/pdb4cr0/pdb
PDB entry 4cr0 coloured by chain, front view.
PDB entry 4cr0 coloured by chain, side view.
PDB entry 4cr0 coloured by chain, top view.
Source organism: Influenza A virus (A/Vietnam/1194/2004(H5N1))
Primary publication:
Enhanced human receptor binding by H5 haemagglutinins.
Xiong X, Xiao H, Martin SR, Coombs PJ, Liu J, Collins PJ, Vachieri SG, Walker PA, Lin YP, McCauley JW, Gamblin SJ, Skehel JJ
Virology 456-457 179-87 (2014)
PMID: 24889237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179550 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 37.07 KDa
Source organism: Influenza A virus (A/Vietnam/1194/2004(H5N1))
Expression system: Gallus gallus
UniProt:
  • Canonical: Q6DQ34 (Residues: 17-342; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 166 amino acids
Theoretical weight: 19.09 KDa
Source organism: Influenza A virus (A/Vietnam/1194/2004(H5N1))
Expression system: Gallus gallus
UniProt:
  • Canonical: Q6DQ34 (Residues: 347-512; Coverage: 29%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P63
Unit cell:
a: 77.95Å b: 77.95Å c: 210.411Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.224 0.262
Expression system: Gallus gallus

Quick links

Citations

7 review citations

The Interplay between the Host Receptor and Influenza Virus Hemagglutinin and Neuraminidase.
Byrd-Leotis et al. (2017)
6 more

1 mention without citation

Molecular Docking of Broad-Spectrum Antibodies on Hemagglutinins of Influenza A Virus.
Le et al. (2019)