› 4cro

X-ray diffraction
3.9Å resolution

PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX

Released:
DOI: 10.2210/pdb4cro/pdb
PDB entry 4cro coloured by chain, front view.
PDB entry 4cro coloured by chain, side view.
PDB entry 4cro coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex.
Brennan RG, Roderick SL, Takeda Y, Matthews BW
Proc Natl Acad Sci U S A 87 8165-9 (1990)
PMID: 2146682

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117298 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein cro Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 66 amino acids
Theoretical weight: 7.38 KDa
Source organism: Escherichia virus Lambda
UniProt:
  • Canonical: P03040 (Residues: 1-66; Coverage: 100%)
Gene names: cro, lambdap57
Sequence domains: Cro
DNA (5'-D(*TP*AP*TP*CP*AP*CP*CP*GP*CP*GP*GP*GP*TP*GP*AP*TP*A)-3') Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 17 nucleotides
Theoretical weight: 5.23 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P32
Unit cell:
a: 154.8Å b: 154.8Å c: 86Å
α: 90° β: 90° γ: 120°
Expression system: Not provided

Quick links

Citations

10 review citations

The TetR family of transcriptional repressors.
Ramos et al. (2005)
9 more

5 mentions without citation

Studying protein-DNA interactions using atomic force microscopy.
Beckwitt et al. (2018)
4 more