› 4crx

X-ray diffraction
2.2Å resolution

ASYMMETRIC DNA-BENDING IN THE CRE-LOXP SITE-SPECIFIC RECOMBINATION SYNAPSE

Released:
DOI: 10.2210/pdb4crx/pdb
PDB entry 4crx coloured by chain, front view.
PDB entry 4crx coloured by chain, side view.
PDB entry 4crx coloured by chain, top view.
Source organism: Punavirus P1
Primary publication:
Asymmetric DNA bending in the Cre-loxP site-specific recombination synapse.
Guo F, Gopaul DN, Van Duyne GD
Proc Natl Acad Sci U S A 96 7143-8 (1999)
PMID: 10377382

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117299 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Recombinase cre Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 36.38 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06956 (Residues: 20-341; Coverage: 94%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
DNA (35 NUCLEOTIDE CRE RECOGNITION SITE) Chains: C, D
Molecule details ›
Chains: C, D
Length: 35 nucleotides
Theoretical weight: 10.76 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2221
Unit cell:
a: 108.8Å b: 122.1Å c: 181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Protein production and purification.
Structural Genomics et al. (2008)
12 more

16 mentions without citation

Asymmetric DNA bending in the Cre-loxP site-specific recombination synapse.
Guo et al. (1999)
15 more